Perthshire Amateur Football Association 2009-10
| Team | Usual Home Venue | Colours |
| Alyth AFC | Diamond Jubilee Park, Alyth | (H): Violet / White (A): |
| Auchterarder AFC | Public Park, Auchterarder | (H):
All Black (A): Yellow / Blue |
| Ballinluig AFC | Recreation Ground, Ballinluig | (H):
Blue / Red (A): |
| Balmoral United AFC | Davie Park, Blairgowrie | (H):
Yellow / Black (A): Navy / Red |
| Breadalbane AFC | Home Street, Aberfeldy | (H):
Blue (A): Red / Black |
| Bridge of Earn AFC | Victory Park, Bridge of Earn | (H): Sky
Blue / Claret (A): Royal Blue / White |
| Bridgeton United AFC | Recreational Park, Almondbank | (H): All
White (A): Red / Black |
| Burrelton Rovers AFC | Recreation Park, Burrelton | (H): Red &
White (A): Dark Blue |
| Comrie Rovers AFC | Laggan Park, Comrie | (H): Black / White (A): Yellow / Black |
| Coupar Angus AFC | Public Park, Coupar Angus | (H): Yellow (A): Maroon |
| Craigie AFC | South Inch Pitch 2, Perth | (H): Blue / White (A): |
| Errol AFC | Errol Public Park, Errol | (H):
Red / White / Black (A): Purple / White |
| Fairfield AFC | South Inch Pitch 5, Perth | (H): Red / White (A): |
| Jeanfield Swifts AFC | Riverside Park, Perth | (H):
Blue & White Stripes (A): Red & Black Stripes |
| Kettins AFC | Lindsay Park, Kettins | (H):
Red / Black (A): |
| Kenmore AFC | Recreation Park, Kenmore | (H):
Red / White (A): Bolue / White |
| Kinrossie Caledonian AFC | Kinrossie | (H):
Navy (A): Red / Black |
| Letham AFC | Seven Acres, Perth | (H):
Tangerine (A): White / Tangerine |
| Letham United AFC | Seven Acres, Perth | (H): Yellow / Blue (A): Red / White |
| Letham Lads AFC | South Inch, Perth | (H):
White / Orange (A): Orange / Black |
| Meigle Vics AFC | Victory Park, Meigle | (H):
Maroon / Blue (A): |
| Methven AFC | King George IV Playing Field, Methven | (H): Red
/ Black (A): Green |
| Newtyle Hearts AFC | Newtyle Park, Newtyle | (H): Red / Black (A): Yellow / Black |
| Perth Villareal AFC | South Inch Pitch 4, Perth | (H): Yellow
/ Blue (A): Red / Black |
| Rattray AFC | Davie Park, Blairgowrie | (H):
Red / Black (A): Yellow / Black |
| Roselea AFC | South Inch Pitch 1, Perth | (H): White / Royal
Blue (A): Royal Blue / White |
| St. Johns AFC | North Inch Pitch 15, Perth | (H): Maroon /
Sky Blue (A): Sky Blue / Maroon |
| Star of Atholl AFC | Blair Atholl | (H):
Yellow / Black (A): |
| Strathearn Grove AFC | Braidhaugh, Crieff | (H):
Sky Blue / Maroon (A): Yellow / Blue |
| Taybank AFC | North Inch Pitch 13, Perth | (H): Blue & White (A): |
| Tay Thistle AFC | Jeanfield Recreation Ground (The Bowl), Perth | (H):
Red / Black (A): Tangerine / Black |
| Vale of Atholl AFC | Recreation Park, Pitlochry | (H): White
/ Black (A): Tangerine / Black |
| Vale of Earn AFC | Market Park, Crieff | (H): Black / White (A): Red / Black |
| Wolfhill AFC | Guildtown Hall, Guildtown | (H):
Black / White (A): Blue / White |